Journal: Frontiers in immunology

Article Title: ChemR23 activation reprograms macrophages toward a less inflammatory phenotype and dampens carcinoma progression.

doi: 10.3389/fimmu.2023.1196731

Figure Lengend Snippet: FIGURE 4 CMKLR1 expression in breast cancer and mesothelioma tumors correlates to TAM markers and ChemR23 is expressed in in vitro TAM-like models. Correlation of CMKLR1 expression with macrophage markers CD14, CD163, MRC1 and HLA-DRA in breast cancers (n=1034) (A) and mesothelioma (n=87) (B) tumors using transcriptomic data from the TCGA database. (C) IHC staining of ChemR23 and Iba1 in breast tumors and MPM from 9 patients in tumor and peritumoral areas. (D) Quantification of ChemR23 IHC staining by HPF (high power field, 1590mm2) for each patient.

Article Snippet: Samples were then incubated during 32min at 37°C with the antiChemR23 antibody at a dilution of 1:200 (clone 1A7, mouse monoclonal, Origene) and with the anti-Iba1 antibody at a dilution of 1:1000 (clone EPR16588, rabbit monoclonal, Abcam).

Techniques: Expressing, In Vitro, Immunohistochemistry

Journal: Mediators of Inflammation

Article Title: Lipopolysaccharide Sensitizes Steroid-Induced Brain Injury in Neonatal Rat Pups

doi: 10.1155/mi/8285898

Figure Lengend Snippet: Determination of TUNEL-positive cells. Sections from three brain regions (hippocampus, cerebellum, and brain stem) were stained using NeuN, GFAP, Iba1, and SOX10 to identify neurons, astrocytes, microglia, and oligodendrocyte precursor cells, respectively in TUNEL + cells. (A–D) Representative immunofluorescent micrographs of dual NeuN + /TUNEL + (A), GFAP + /TUNEL + (B), Iba1 + /TUNEL + (C), and SOX10 + /TUNEL + (D) cells. White arrows indicate dual positive stained cells. DAPI staining was used to confirm the presence of cell nuclei. (E–G) Quantitative results on TUNEL-positive cell types presented in pie charts for three different brain regions.

Article Snippet: After TUNEL stain, brain sections blocked with 3% normal goat serum (Cat# S26-M, Sigma-Aldrich, St. Louis, MO) were prepared in phosphate-buffered sal with Tween 20 (PBST) for 1 h at room temperature and probed with one of the following primary antibodies: mouse antineuronal nuclear antigen (NeuN) (1:250 dilution, Cat. # MAB377, Merck KGaA), rabbit antiglial fibrillary acidic protein (GFAP) (1:250 dilution, Cat. # Z0334, Dako-Agilent, Santa Clara, CA), rabbit anti-ionized calcium-binding adapter molecule-1 (Iba1) (1:250, Cat. #: 019-19741, Wako Pure Chemical Industries, Osaka, Japan), and rabbit anti-SRY-box transcription factor 10 (SOX10) (1:250 dilution, Cat. #: TA381887, Ori-Gene, Rockville, MA) for 16 h at room temperature.

Techniques: TUNEL Assay, Staining